Finization versant kpcr manual protocol on a VERSANT® kPCR Sample Prep Machine according to the protocol supplied by the ma-nufacturer (Siemens Healthcare Diagnostics, Breda, The Netherlands). The VERSANT HIV-1 RNA 1. J Clin Virol 44(4): 297–301. View Article Google Scholar 40.
Versant Systems introduces S-DOES E-sign & Digital Signature platform for digitally transforming organizations. The VERSANT kPCR Molecular System with the MiPLX Software Solution is from Siemens. The ProbeTec needs more than 1. For real-time PCR, performed on the VERSANT kPCR amplification and detection unit (Siemens Healthcare Diagnostics, Tarrytown, NY), commercially available, IVD/CE-labeled real-time PCR assays were employed. VERSANT kPCR Molecular System SPVERSANT kPCR Molecular System AD Published by Siemens Healthcare Diagnostics Inc.
The VERSANT® Zika RNA 1. Versant kPCR Molecular System (Siemens Healthcare, Erlangen, Germany) according to the following protocol: 5 min at 50 ° C, 20 sec at 95 ° C followed by 40 cycles of 15 sec at 95 ° C and 60 s at 60 ° C and according to MammaTyper® instructions for useEU. VERSANT® kPCR Molecular System AD. FTD kits work with a large range of automated and manual extraction kits. VERSANT ® kPCR Molecular System AD ABI Prism ® 7500 SDS and versant kpcr manual 7500 Fast SDS LightCycler ® 480 Instrument II Rotor-Gene ® 3000/6000 Rotor-Gene ® Q 5/6 plex Platfom CFX96™ Real-Time PCR Detection System CFX96™ Deep Well Real-Time PCR Detection System. Applied Biosystems ® 7500/7500 Fast, ViiA™7, QuantStudio™5 (Life Technologies™) Rotor-Gene 3000/6000/Rotor-Gene Q (Qiagen) CFX96™ (Bio-Rad) LightCycler ® 480 (Roche).
Perform the manual baseline adjustment as described in the previous section (Fig. Contact us for more details. The VERSANT ® kPCR Molecular System * Not available for sale in the U. “Four or five years ago, NGS was incredibly manual,” says Felton, “but now it comes with quite simple workflows so that almost anyone can adopt the technology. Get repeatable and solid results – every time. , aseptic meningitis, paralysis, myocarditis, and neonatal enteroviral sepsis).
0 kPCR assay (kPCR) and Roche CAP/CTM Quantitative test v2. System flexibility in combination with an expanding menu will support quantitative and qualitative detection across many molecular diagnostic applications. To view these files, download the following free software. The VERSANT® kPCR Sample Prep with the MiPLX Software Solution provides complete flexibility and expands the capability of your molecular testing including automation of your own assays.
The unique Siemens universal extraction technology provides high-quality nucleic acids from diverse specimens. Under the terms of the agreement, its capabilities will expand versant kpcr manual to include FTD’s CE-marked kits and multisyndromic panels, which cover conditions ranging from childhood infections to gastroenteritis. This study compares VL values using Siemens VERSANT HIV-1 RNA 1. It is an automated amplification method based on reverse transcription and kPCR. The VERSANT® kPCR Molecular System provides your laboratory with a semi - automated system combining a fully automated Sample Preparation (SP) module for the isolation and purification of nucleic acids, and separately, a kpcr fully automated Amplification and Detection (AD) for nucleic acids using kPCR technology, linked by software. The VERSANT®kPCR Molecular System is an automated solution that provides standardized sample extraction, flexible open-channel capabilities for laboratory-developed and third-party assays, and quality assays for infectious-disease testing. VERSANT kPCR Molecular System. The Siemens VERSANT kPCR Sample Prep system, Abbott msp platform, and Qiagen Qiasymphony SP/AS (Qiagen, Venlo, the Netherlands) are stand-alone liquid handling robots that automate nucleic acid extraction and assay setup, wherein final eluates are combined with master-mix reagents.
VERSANT kPCR molecular systems SP: Manual isolation:. 0 kPCR molecular system is the latest real-time assay approved for HIV monitoring. In this study, the performance of the VERSANT HIV-1 RNA 1. 5 hours for wet swabs and almost three (3) hours for urines. 0 kinetic PCR molecular system (kPCR) for the quantification of HIV-1 plasma viral load. 0) in 176 DBS carrying different HIV-1 variants collected. · Manual processing of DBS was minimal, with an additional 30 min incubation prior to automated sample preparation on an unmodified VERSANT kPCR Molecular System. Versant kPCR: UPC: Does Not Apply: Bullseye Industrial Sales.
Switch on the Stratagene Mx3000P/3005P or on the Versant kPCR. Document Library - Siemens Healthineers. ®Siemens Versant kPCR Cycler ® Bio-Rad CFX96 (IVD, non-deep well) Agilent Technologies Mx3000P MammaTyper® Kit Facts Designed for 10 determinations (max. Manual Automated: QIAamp DNA Blood Mini kit QIAcube NucliSENS ® easyMAG ® MagNA Pure Compact MagNA Pure LC MagNA Pure 96 msp VERSANT kPCR Molecular System SP QIAsymphony SP: Validated Amplification platform* Life Technologies (Applied Biosystems) LightCycler 1. Each DNA-isolation run included a negative control (HPLC-grade water). 0 is a manual in vitro diagnostic test, based on real-time PCR technology, for the. Metric 4: Manual / Hands-on time The kPCR platform requires ~45 minutes of hands-on time (manual + vigilant) and the m ~54 minutes. · Ruelle J, Jnaoui K, Lefevre I, Lamarti N, Goubau N () Comparative evaluation of the VERSANT HIV-1 RNA 1.
Graph 5 summarizes the results for this metric. Each sample was centrifuged at 15,000g for one minute, then 700 μl buffer consisting. manual extraction (High Pure System Viral Nucleic Acid Kit) conserved 5´UTR and core region 1a, 1b, 2a,2b, 3, 4, 5 and 6. View and Download Agilent Technologies Mx3000P setup and user manual online. The new version of the kPCR VERSANT assay also seems reliable for the quantification of HIV-1 group O variants. Enteroviruses are common human viruses associated with diverse clinical syndromes ranging from minor febrile illness to rare but severe and potentially fatal conditions (e. Validated Extraction platform* Manual Automated: QIAamp DNA Blood Mini kit NucliSENS ® easyMAG ® MagNA Pure Compact MagNA Pure LC MagNA Pure 96 QIAcube QIAsymphony SP : msp VERSANT kPCR Molecular System SP: Validated Amplification platform*. Enterovirus R-GENE ® advantages.
• ®VERSANT kPCR Molecular System AD (Siemens Healthcare) • ®ABI Prism 7500 SDS (Applied Biosystems) • ABI Prism® 7500 Fast SDS (Applied Biosystems) • Rotor-Gene® 6000 (Corbett Research) • Rotor-Gene® Q5/6 plex Platform (QIAGEN) • CFX96™ Real-Time PCR Detection System (Bio-Rad). 0 Assay (LiPA) is a line probe assay, for in vitro diagnostic use, which identifies Hepatitis C virus (HCV) genotypes 1 to 6 and subtypes 1a and 1b in human serum or. 0 Assay (kPCR) Kit is a real-time PCR based assay intended for the qualitative detection of RNA from the Zika virus in serum, EDTA plasma and urine (collected alongside a. 0 Assay (kPCR) was evaluated and compared to the COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, version 2.
Product availability varies from versant country to country and is subject to local regulatory requirements The GeneXpert System is a closed, self-contained, fully-integrated and automated platform that provides results in a timely manner with minimal risk of contamination. Skilled analysts can be redeployed to other demanding tasks such as data analysis. 30-20-DX-291-76 ·· ©Siemens Healthcare Diagnostics Inc. Compatible Real-Time PCR instruments. 0 Assay (kPCR) Kit is a real-time PCR (RT-PCR) assay for the qualitative detection of RNA from Zika virus in serum, EDTA plasma, and urine (collected alongside a. Mx3000P Laboratory Equipment pdf manual download. · The VERSANT®Zika RNA 1.
VERSANT® kPCR Molecular System AD (Siemens) ABI Prism® 7500 SDS (Applied Biosystems) ABI Prism® 7500 Fast SDS (Applied Biosystems) Rotor-Gene® 6000 (Corbett Research) Rotor-Gene® Q5/6 plex Platform (QIAGEN) CFX96™ Real-Time System (Bio-Rad) CFX96™ Deep Well Real-Time System (Bio-Rad) LightCycler® 480 Instrument II (Roche. Graph 5: Manual Time kPCR M ProbeTec ProbeTec ‐Urine. · Hamilton Micro Lab Microlab 1000 Diluter Dispenser w/ Manual Power cable & Gun.
The RealStar® Filovirus Screen RT-PCR Kit 1. Biotage® Extrahera™ automates sample processing and does it with engineered precision. • ®VERSANT kPCR Molecular System SP (Siemens Healthcare) Alternative nucleic acid extraction systems and kits might also be appropriate.
The Versant system uses an open-channel design and Siemens’ customizable MiPLX software to reduce the need for manual labor. Employing proprietary extraction technology that supports multiple sample types, our VERSANT ® kPCR Molecular System* delivers accurate, reliable results with maximum productivity. No More Tedious Manual Labor. · However, the level of integration varies among these platforms.
The Best of Both Worlds. 8 patient samples) Easy-to-run assay protocol Reduced hands-on time for pathologists MammaTyper® delivers results from FFPE sample to report within 6 hours RNXtract® RNA extraction. Get Adobe® Reader® to view PDF&39;s © Versant. If using a spin column based sample preparation procedure including washing buffers containing ethanol, it is highly recommended to perform an additional.
Validated Extraction platform* Manual Automated: QIAamp DNA Blood Mini kit NucliSENS ® easyMAG ® MagNA Pure Compact MagNA Pure LC QIAcube QIAsymphony SP msp VERSANT kPCR Molecular System SP: Validated Amplification platform* The VERSANT®HCV Genotype 2. Funding No external funding, apart from the support of the authors’ institution, was available for this study. Some content on this website is saved in PDF format. · After nucleic acid extraction employing protocols as recommended by the manufacturers, all eluates were frozen at −70 °C until analyzed.
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